microcapillary holder (Narishige inc)
Structured Review
Microcapillary Holder, supplied by Narishige inc, used in various techniques. Bioz Stars score: 97/100, based on 1246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microcapillary+holder/Microinjector/pm37120634-303-8-16
Average 97 stars, based on 1246 article reviews
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Single Cell:Article Title: Single-cell metabolic fingerprints discover a cluster of circulating tumor cells with distinct metastatic potential. Article Snippet: The pulled capillaries were further processed with a Microforge (MF2, Narishige, Tokyo, Japan) to make the holders ∼1μm open. .. Single-cell micro-sampling and single-cell quantitative mass spectrometry The Mass Spectrometry:Article Title: Single-cell metabolic fingerprints discover a cluster of circulating tumor cells with distinct metastatic potential. Article Snippet: The pulled capillaries were further processed with a Microforge (MF2, Narishige, Tokyo, Japan) to make the holders ∼1μm open. .. Single-cell micro-sampling and single-cell quantitative mass spectrometry The Extraction:Article Title: Single-cell metabolic fingerprints discover a cluster of circulating tumor cells with distinct metastatic potential. Article Snippet: The pulled capillaries were further processed with a Microforge (MF2, Narishige, Tokyo, Japan) to make the holders ∼1μm open. .. Single-cell micro-sampling and single-cell quantitative mass spectrometry The Article Title: Single-cell metabolic fingerprints discover a cluster of circulating tumor cells with distinct metastatic potential Article Snippet: The pulled capillaries were further processed with a Microforge (MF2, Narishige, Tokyo, Japan) to make the holders ∼1 μm open. .. The Injection:Article Title: Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA Article Snippet: We previously reported mRNA caging technology as a novel and simple technique for photo-mediated temporal and spatial control of gene activation in zebrafish embryos and as an alternative to the ‘gene knockdown’ approach using antisense morpholino oligonucleotides.. The caging reagent used is 6-bromo-4-diazomethyl-7-hydroxycoumarin (Bhc-diazo), which forms a covalent bond with the phosphate moiety of the sugar-phosphate backbone of RNA.. Mainly because of the reduced solubility Transferring:Article Title: Enhancer detection in the zebrafish using pseudotyped murine retroviruses. Article Snippet: Vectors based on murine retroviruses are among the most eYcient means to insert reporter constructs into the context of a vertebrate chromosome with the aim to visualize cis-regulatory information available to a basal promoter at the site of insertion.. In combination with using the zebraWsh embryo as a readout for the activity of regulatory elements, enhancer detection becomes a powerful technique for gene discovery and for the mapping of the extent of regulatory domains in a vertebrate genome.. Our laboratory has performed the only large-scale enhancer detection screen to date in any vertebrate and we describe in this paper the methods we developed to generate viral particles, to insert reporter constructs into the zebraWsh germ line, the screening of detection events in heterozygous F1 embryos, and the isolation of genomic sequence Xanking the inserted vector for the purpose of genomic mapping. Microscopy:Article Title: Enhancer detection in the zebrafish using pseudotyped murine retroviruses. Article Snippet: Vectors based on murine retroviruses are among the most eYcient means to insert reporter constructs into the context of a vertebrate chromosome with the aim to visualize cis-regulatory information available to a basal promoter at the site of insertion.. In combination with using the zebraWsh embryo as a readout for the activity of regulatory elements, enhancer detection becomes a powerful technique for gene discovery and for the mapping of the extent of regulatory domains in a vertebrate genome.. Our laboratory has performed the only large-scale enhancer detection screen to date in any vertebrate and we describe in this paper the methods we developed to generate viral particles, to insert reporter constructs into the zebraWsh germ line, the screening of detection events in heterozygous F1 embryos, and the isolation of genomic sequence Xanking the inserted vector for the purpose of genomic mapping. Sterility:Article Title: Enhancer detection in the zebrafish using pseudotyped murine retroviruses. Article Snippet: Vectors based on murine retroviruses are among the most eYcient means to insert reporter constructs into the context of a vertebrate chromosome with the aim to visualize cis-regulatory information available to a basal promoter at the site of insertion.. In combination with using the zebraWsh embryo as a readout for the activity of regulatory elements, enhancer detection becomes a powerful technique for gene discovery and for the mapping of the extent of regulatory domains in a vertebrate genome.. Our laboratory has performed the only large-scale enhancer detection screen to date in any vertebrate and we describe in this paper the methods we developed to generate viral particles, to insert reporter constructs into the zebraWsh germ line, the screening of detection events in heterozygous F1 embryos, and the isolation of genomic sequence Xanking the inserted vector for the purpose of genomic mapping. other:Article Title: Use of chick neural tube for optimizing the PSM and epithelial somites electroporation parameters: A detailed protocol Article Snippet: Fill in the microcapillary with DNA/Fast Green mix or fluorescent CMO using Eppendorf microloader fine tip and then mount the microcapillary onto the microcapillary holder. |

